Journal: Stem cells (Dayton, Ohio)

Article Title: Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment.

doi: 10.1002/stem.1023

Figure Lengend Snippet: Figure 6. Significant upregulation of human HGF in conditioned medium was confirmed by ELISA. HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.

Article Snippet: Test and control media (DMEM or SMIM media exposed to hASC at time 0) were collected and examined for hepatocyte growth factor (HGF) levels using the Quantikine Human HGF ELISA kit (R&D Systems, Minneapolis, MN, www.rndsystems. com) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Staining, Injection, Expressing

Journal: Scientific Reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Secretion of growth factors by adipose-derived stem cells (ASCs) and the formation of networks by co-culture with endothelial cells (ECs) or lymphatic endothelial cells (LECs). ( a ) The levels of vascular endothelial growth factor-C (VEGF-C), VEGF-A, hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in the medium bathing cultured ASCs were measured using enzyme-linked immunosorbent assays. HGF and VEGF-A were both secreted by ASCs, whereas the levels of secreted VEGF-C and b-FGF were much lower. ( b ) Green fluorescent protein (GFP)-expressing ECs and ASCs co-cultured at a ratio of 1:4. Networks of vascular ECs were observed, and the edges of the vascular EC network were sharp (arrows). ( c ) GFP-LECs and ASCs co-cultured at a ratio of 1:4. The LECs formed networks, but in contrast to vascular ECs, rounded/bulging structures were observed at the edges of the LEC network (arrowheads). ( d ) Merge image of ( c ) and phase-contrast image. ASCs shows random pattern with no specific character which do not form own network or support LEC network directly.

Article Snippet: The concentrations of VEGF-C, VEGF-A, HGF, and bFGF were measured by enzyme-linked immunosorbent assay (ELISA) using a Human VEGF-C ELISA Kit (P49767, RayBiotech, USA), LBIS Human VEGF ELISA Kit (631-40831, Fujifilm Wako, Japan), AuthentiKine Human HGF ELISA Kit (KE00168, Proteintech, USA) and Human bFGF ELISA Kit (P09038, RayBiotech, USA) in accordance with the manufacturers’ instructions.

Techniques: Derivative Assay, Co-Culture Assay, Cell Culture, Expressing

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Pre- and post-extraction OPG, RANKL, HGF, TNF-α, IL-18, MMP-9, TOS—total oxidant status, TAC—total antioxidant capacity, and HbA1c values in control and diabetes mellitus patients.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Control

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: ROC curve. Prediction of salivary markers in the determinism of HbA1c > 6.5%.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques:

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Post-extraction correlation of HbA1c with salivary markers (OPG, RANKL, OPG/RANKL, HGF, TNF-α, MMP-9, IL-18, TOS and TAC).

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Extraction

Journal: International Journal of Molecular Sciences

Article Title: Blockade of c-Met-Mediated Signaling Pathways by E7050 Suppresses Growth and Promotes Apoptosis in Multidrug-Resistant Human Uterine Sarcoma Cells

doi: 10.3390/ijms232314884

Figure Lengend Snippet: Effects of E7050 on the expression and secretion of HGF in MES-SA/Dx5 cells. ( A ) Cells were treated with various concentrations (5–25 μM) of E7050 for 24 h. Whole cell extracts were prepared and subjected to Western blotting using antibodies against HGF and β-actin. β-actin was used as an internal loading control. ( B ) Densitometric analysis of blots relative to HGF protein after normalization with β-actin. ( C ) Secreted HGF in cell culture media was determined by ELISA. Data are presented as the mean ± SEM of three independent experiments. * p < 0.05 versus vehicle-treated control cells.

Article Snippet: The concentrations of HGF in E7050-treated MES-SA/Dx5 culture medium were measured using a human HGF ELISA Kit PicoKine TM according to the manufacturer’s instructions (Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Expressing, Western Blot, Control, Cell Culture, Enzyme-linked Immunosorbent Assay